Abstract
Cell immobilization is an effective method to prolong the life time of microorganism and has been proved their feasibility in some other biosensors. Thus, we study on the use of Escherichia coli (E. coli) immobilized by agar, gelatin, agar-gelatin mixture (agar/gelatin), chitosan and polyvinyl alcohol (PVA) to screen toxicity electrochemically. The E. coli immobilized by PAV gel showed the highest apparent bioactivity and the longest storage time in pH 7.0 PBS solution. Furthermore, the E. coli immobilized by different gels was applied in the toxicity determination via a reported ferricyanide-mediated electrochemical method, where 3,5-dichlorophenol (DCP) was used as a model toxin. E. coli immobilized by PVA showed the highest sensitivity to DCP and the corresponding value of 50% inhibition concentration (IC50) was 9.62 mg L−1. The values of IC were in the range of 6.32% and 13.75% when the E. coli immobilized by PAV was challenged by wastewater, which were comparable with those obtained with the standard luminescent bacterial method (value of EC was in the range of 7.96%–25.42% for the same samples). Given the apparent bioactivity, storage ability and sensitivity to toxin, PVA was the best polymer to confine cells among the polymers used in this work. This article is protected by copyright. All rights reserved
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