Abstract
The zebrafish (Zf) Fish Embryo Toxicity (FET) test is increasingly employed for alternative toxicity studies, yet our recent research identified increased sensitivity of Zf slightly older than embryos employed in FET methods (0–4 dpf, days post fertilization). Here, we identified rapid steady-state accumulation of diphenhydramine across Zf embryo and larval stages. However, significantly (p < 0.05) lower accumulation was observed in chorionated and dechorionated embryos (0-4 dpf), but not in 7–11 and 14–18 dpf Zf, at 48 hours than 96 hours. Increased uptake of diphenhydramine was further observed in 7–11 and 14–18 dpf Zf compared to 0-4 dpf embryos with chorion or dechorionated, which suggests differential Zf sensitivity with age is associated with accumulation resulting from gill and other toxicokinetic and toxicodynamic changes during development. This article is protected by copyright. All rights reserved
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