UPTAKE OF RADIOLABELED 3,3',4,4'-TETRACHLOROBIPHENYL INTO JAPANESE QUAIL EGG COMPARTMENTS AND EMBRYO FOLLOWING AIR CELL AND ALBUMEN INJECTION

Abstract

The avian embryo is an excellent model for testing adverse developmental effects of environmental chemicals, and uptake and movement of xenobiotics within the egg compartments. ¹⁴C 3,3',4,4'-tetrachlorobiphenyl (¹⁴C PCB 77) was injected into Japanese quail eggs prior to incubation at embryonic day 0 (ED0) either onto the air cell or into the albumen. All egg components were collected on ED1, 5 or 10 and concentrations of ¹⁴C PCB 77 were measured in various egg components (shell, membrane, yolk, albumen and embryo). Results showed measurable ¹⁴C PCB 77 in all egg components, with changing concentrations in each egg component over the course of embryonic development. Specifically, concentrations in the shell content decreased between ED1-ED10, increased in albumen from ED1 to ED5 and then decreased at ED10, and increased in both yolk and embryo increased from ED1 to ED10. Vehicle and injection site both influenced ¹⁴C PCB 77 allantoic fluid concentrations, with little effect on other egg components except for the inner shell membrane. The fatty acid vehicle injected into the albumen yielded the highest ¹⁴C PCB 77 recovery. These findings demonstrate dynamic movement of toxicants throughout the egg components during avian embryonic development and a steady increase of relatively low levels of ¹⁴C PCB 77 in the embryo compared to yolk, albumen and shell, suggesting that embryonic uptake (i.e., exposure) mirrors utilization of egg components for nutrition and growth during development. This article is protected by copyright. All rights reserved

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